Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured illumination microscopy

Nurmohammed Patwary; Jorge Sola-Pikabea; Ana Doblas; Genaro Saavedra; Manuel Martínez-Corral; Chrysanthe Preza

doi:10.1117/12.2291090

23 February 2018 Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured illumination microscopy

Nurmohammed Patwary, Jorge Sola-Pikabea, Ana Doblas, Genaro Saavedra, Manuel Martínez-Corral, Chrysanthe Preza

Author Affiliations +

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 104991A (2018) https://doi.org/10.1117/12.2291090
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Three-dimensional imaging is affected by depth-induced spherical aberration (SA) when imaging deep into an optically thick sample. In this work, we evaluate the impact of SA on the performance of incoherent grating-projection structured illumination microscopy (SIM). In particular, we analyze the reduction of the contrast in the structured pattern and compare the reconstructed SIM images for different amounts of SA. In order to mitigate the impact of SA, we implement and evaluate in SIM a wavefront encoded imaging system using a square cubic (SQUBIC) phase mask, an approach shown previously to be successful in conventional microscopy.

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Nurmohammed Patwary, Jorge Sola-Pikabea, Ana Doblas, Genaro Saavedra, Manuel Martínez-Corral, and Chrysanthe Preza "Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured illumination microscopy", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991A (23 February 2018); https://doi.org/10.1117/12.2291090

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