Quantitative phase imaging characterization of tumor-associated blood vessel formation on a chip

Peng Guo; Jing Huang; Marsha A. Moses

doi:10.1117/12.2289006

23 February 2018 Quantitative phase imaging characterization of tumor-associated blood vessel formation on a chip

Peng Guo, Jing Huang, Marsha A. Moses

Proceedings Volume 10503, Quantitative Phase Imaging IV; 105031O (2018) https://doi.org/10.1117/12.2289006
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Angiogenesis, the formation of new blood vessels from existing ones, is a biological process that has an essential role in solid tumor growth, development, and progression. Recent advances in Lab-on-a-Chip technology has created an opportunity for scientists to observe endothelial cell (EC) behaviors during the dynamic process of angiogenesis using a simple and economical in vitro platform that recapitulates in vivo blood vessel formation. Here, we use quantitative phase imaging (QPI) microscopy to continuously and non-invasively characterize the dynamic process of tumor cell-induced angiogenic sprout formation on a microfluidic chip. The live tumor cell-induced angiogenic sprouts are generated by multicellular endothelial sprouting into 3 dimensional (3D) Matrigel using human umbilical vein endothelial cells (HUVECs). By using QPI, we quantitatively measure a panel of cellular morphological and behavioral parameters of each individual EC participating in this sprouting. In this proof-of-principle study, we demonstrate that QPI is a powerful tool that can provide real-time quantitative analysis of biological processes in in vitro 3D biomimetic devices, which, in turn, can improve our understanding of the biology underlying functional tissue engineering.

Citation Download Citation

Peng Guo, Jing Huang, and Marsha A. Moses "Quantitative phase imaging characterization of tumor-associated blood vessel formation on a chip", Proc. SPIE 10503, Quantitative Phase Imaging IV, 105031O (23 February 2018); https://doi.org/10.1117/12.2289006

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