Interrogation of evolving tumor vasculature using high-resolution CT imaging and a nanoparticle contrast agent

Ketan B. Ghaghada; Zbigniew Starosolski; Igor Stupin; Poonam Sarkar; Ananth Annapragada

doi:10.1117/12.2293607

12 March 2018 Interrogation of evolving tumor vasculature using high-resolution CT imaging and a nanoparticle contrast agent

Ketan B. Ghaghada, Zbigniew Starosolski, Igor Stupin, Poonam Sarkar, Ananth Annapragada

Author Affiliations +

Proceedings Volume 10578, Medical Imaging 2018: Biomedical Applications in Molecular, Structural, and Functional Imaging; 105781C (2018) https://doi.org/10.1117/12.2293607
Event: SPIE Medical Imaging, 2018, Houston, Texas, United States

Abstract

The architecture of intra-tumoral vascular network continuously evolves with tumor progression. Non-invasive methods that facilitate 3D in vivo interrogation of tumor vascular architecture could improve understanding of tumor progression and metastasis. In this work, we studied evolving tumor vasculature using high-resolution CT images and a blood-pool, nanoparticle iodinated contrast agent. In vivo studies were performed in a transgenic mouse model of neuroblastoma that exhibit spontaneous bilateral tumors in the adrenals. Animals were divided into three groups based on tumor age: early-age tumor, intermediate-age tumor, old-age tumor. Tumor progression was monitored using T2-weighted MRI. Contrast-enhanced CT imaging was performed at two points: the first imaging session (leak map) was performed 4 days after administration of the nanoparticle agent to interrogate changes in tumor vascular permeability. Immediately thereafter, a second dose of contrast agent was administered and CT imaging was performed within 1 hour to capture high-resolution angiograms of tumor vasculature. CT angiograms demonstrated the highly-vascularized nature of these tumors. Old-age tumors exhibited a higher fractional volume of avascular regions and an increased number of large superficial blood vessels on tumor periphery. Old-age tumors also demonstrated the presence of intra-vessel tumor thrombus and the invasion of tumor into the inferior vena cava. Leak maps images demonstrated signal enhancement throughout the tumor in early-age tumors, including the core region, suggestive of the presence of highly permeable blood vessels through the tumor volume. Old-age tumors exhibited relatively lower signal enhancement, indicative of a less 'leaky' tumor vascular network compared to early and intermediate-stage tumors.

Citation Download Citation

Ketan B. Ghaghada, Zbigniew Starosolski, Igor Stupin, Poonam Sarkar, and Ananth Annapragada "Interrogation of evolving tumor vasculature using high-resolution CT imaging and a nanoparticle contrast agent", Proc. SPIE 10578, Medical Imaging 2018: Biomedical Applications in Molecular, Structural, and Functional Imaging, 105781C (12 March 2018); https://doi.org/10.1117/12.2293607

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