Examining structural changes in diabetic nephropathy using inter-nuclear distances in glomeruli: a comparison of variously automated methods

Olivier Simon; Rabi Yacoub; Sanjay Jain; John E. Tomaszewski; Pinaki Sarder

doi:10.1117/12.2295225

6 March 2018 Examining structural changes in diabetic nephropathy using inter-nuclear distances in glomeruli: a comparison of variously automated methods

Olivier Simon, Rabi Yacoub, Sanjay Jain, John E. Tomaszewski, Pinaki Sarder

Author Affiliations +

Proceedings Volume 10581, Medical Imaging 2018: Digital Pathology; 105810B (2018) https://doi.org/10.1117/12.2295225
Event: SPIE Medical Imaging, 2018, Houston, Texas, United States

Abstract

In diabetic nephropathy (DN), hyperglycemia drives a progressive thickening of and damage to the glomerular filtration surfaces, as well as mesangial expansion and a constriction of capillary lumens. This leads at first to high blood pressure, increased glomerular filtration and micro-proteinuria, and later (if untreated) to severe proteinuria and end-stage renal disease (ESRD). Though, it is well known that DN is accompanied by marked histopathological changes, the assessment of these structural changes is to a degree subjective and hence varies between pathologists. In this work, we make a first study of glomerular changes in DN from a graph-theoretical and distance-based standpoint, using minimal spanning trees (MSTs) and distance matrices to generate statistical distributions that can potentially provide a “fingerprint” of DN. We apply these tools to detect notable differences between normal and DN glomeruli in both human disease and in a streptozotocin-induced (STZ) mouse model. We also introduce an automated pipeline for rapidly generating MSTs and evaluating their properties with respect to DN, and make a first pass at three-dimensional MST structures. We envision these approaches may provide a better understanding not only of the processes underway in DN progression, but of key differences between actual human disease and current experimental models.

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Citation Download Citation

Olivier Simon, Rabi Yacoub, Sanjay Jain, John E. Tomaszewski, and Pinaki Sarder "Examining structural changes in diabetic nephropathy using inter-nuclear distances in glomeruli: a comparison of variously automated methods", Proc. SPIE 10581, Medical Imaging 2018: Digital Pathology, 105810B (6 March 2018); https://doi.org/10.1117/12.2295225

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