AOTF-based optical system of a microscope module for multispectral imaging techniques

Olga Polschikova; Alexander Machikhin; Vladislav Batshev; Alina Ramazanova; Artyom Belov; Vitold Pozhar

doi:10.1117/12.2297614

7 December 2017 AOTF-based optical system of a microscope module for multispectral imaging techniques

Olga Polschikova, Alexander Machikhin, Vladislav Batshev, Alina Ramazanova, Artyom Belov, Vitold Pozhar

Proceedings Volume 10592, Biophotonics—Riga 2017; 105920H (2017) https://doi.org/10.1117/12.2297614
Event: The Second International Conference "Biophotonics-Riga 2017", 2017, Riga, Latvia

Abstract

Multi-spectral imaging techniques are widely used in microscopy for many applications. One of the most widespread spectral elements for this purpose is an imaging acousto-optic tunable filter (AOTF). AOTF-based contrast visualization is especially effective when used together with other imaging techniques. Simultaneous utilization of two or more imagers requires optical coupling to provide point-to-point matching of the obtained images. Small linear and angular aperture of AOTF additionally hampers the development of multi-sensor imaging systems for microscopy. In this paper, we present a compact optical system which allows to integrate AOTF-based spectral imager into the schemes of conventional microscopes and provide high-quality spectral image of the same scale as in another, for example, wideband channel. The effectiveness of the proposed approach is demonstrated experimentally.

Citation Download Citation

Olga Polschikova, Alexander Machikhin, Vladislav Batshev, Alina Ramazanova, Artyom Belov, and Vitold Pozhar "AOTF-based optical system of a microscope module for multispectral imaging techniques", Proc. SPIE 10592, Biophotonics—Riga 2017, 105920H (7 December 2017); https://doi.org/10.1117/12.2297614

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