Translator Disclaimer
15 May 2018 Isolation of highly selective phage-displayed oligopeptide probes for detection of listeria monocytogenes in ready-to-eat food
Author Affiliations +
Listeria monocytogenes is the major etiologic agent for foodborne Listeriosis in humans from consumption of readyto- eat (RTE) food. According to Center for Disease Control and Prevention, an estimated 1,600 people contract Listeriosis each year with approximately 260 deaths. This high rate of mortality has alerted the Food Safety Inspection and Services to release the Notice 23-99, Instructions for Verifying the L. monocytogenes Reassessment, on August 3, 1999 for their inspectors. According to the FDA’s Bacterial Analysis Manual Chapter 10, L. monocytogenes in RTE food samples is detected via microbiological culture-based tests, qPCR, pulsed-field gel electrophoresis, and other alternative methods. Unfortunately, these methods are time consuming (48-72 hours) and require dedicated laboratory facility. Thus, to develop a real-time L. monocytogenes biosensor, we isolated L. monocytogenes specific oligopeptides displayed on bacteriophages using modified biopanning procedures. In order to account for major temperature dependent morphological alterations of L. monocytogenes at 4°C versus 37°C, we used bacterial cells adapted to either temperature as the target in our biopanning. To date, we have isolated several candidate probes that can recognize either cold-adapted, warm-adapted L. monocytogenes cells, or both types of bacterial cells. Our isolated probes will be used on the magnetoelastic biosensor platforms for real-time detection of L. monocytogenes in RTE foods stored at 4°C or in samples/fluids for bacterium adapted to human body temperature.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
I-Hsuan Chen, Jianguo Xi, Yuzhe Liu, Songtao Du, Shin Horikawa, Tung-Shi Huang, Bryan A. Chin, and Sang-Jin Suh "Isolation of highly selective phage-displayed oligopeptide probes for detection of listeria monocytogenes in ready-to-eat food", Proc. SPIE 10665, Sensing for Agriculture and Food Quality and Safety X, 106650N (15 May 2018);


New technology for food safety role of the new...
Proceedings of SPIE (March 30 2004)
An automated portable array biosensor
Proceedings of SPIE (July 22 2003)
A portable array biosensor for food safety
Proceedings of SPIE (November 19 2004)
New biosensors for food safety screening solutions
Proceedings of SPIE (May 05 2009)
Detection of E. coli O157 H7 in complex matrices under...
Proceedings of SPIE (February 19 2009)

Back to Top