Fiber Optic Immunochemical Sensors For Continuous Monitoring Of Hapten Concentrations

W. Greg Miller; F. Philip Anderson

doi:10.1117/12.952098

15 June 1989 Fiber Optic Immunochemical Sensors For Continuous Monitoring Of Hapten Concentrations

W. Greg Miller, F. Philip Anderson

Proceedings Volume 1067, Optical Fibers in Medicine IV; (1989) https://doi.org/10.1117/12.952098
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

We describe a fiber optic sensor based on a homogeneous fluorescence energy transfer immunoassay which operates in a continuous, reversible manner to quantitate the anticonvulsant drug phenytoin. B-phycoerythrin-phenytoin and Texas Red labeled anti-phenytoin antibody were sealed inside a short length of cellulose dialysis tubing which was cemented to the distal end of an optical fiber. When the sensor was placed into a solution of phenytoin, the drug crossed the dialysis membrane, displaced a fraction of the B-phycoerythrin-phenytoin from the antibody, and produced a change in fluorescence signal which was measured with a fiber optic fluorometer. The sensor had a concentration response of 5 to 500μmo1/L phenytoin with a response time of 5 to 15 min and precision of <2.5% CV. The chemical kinetics of the antibody-hapten indicator reaction were modeled mathematically and simulation showed that response time in the minutes range can be achieved when the dissociation rate constant is greater than approximately 10^-3 sec^-1. The dissociation rate constant influences the time to reach equilibrium and the unbound P* concentration range available for instrumental measurement. The ratio of the labeled and unlabeled hapten dissociation rate constants influences the analyte concentration range to which the sensor will respond.

Citation Download Citation

W. Greg Miller and F. Philip Anderson "Fiber Optic Immunochemical Sensors For Continuous Monitoring Of Hapten Concentrations", Proc. SPIE 1067, Optical Fibers in Medicine IV, (15 June 1989); https://doi.org/10.1117/12.952098

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