On the use of image quality measures of multi-views in light sheet fluorescence 3D microscopy

Rosa Huaman; Charlotte Riviere; Carole Frindel; Andrea Bassi; David Rousseau

doi:10.1117/12.2305844

24 May 2018 On the use of image quality measures of multi-views in light sheet fluorescence 3D microscopy

Rosa Huaman , Charlotte Riviere, Carole Frindel , Andrea Bassi, David Rousseau

Proceedings Volume 10677, Unconventional Optical Imaging; 106772V (2018) https://doi.org/10.1117/12.2305844
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

In this report, we discuss the interest of quality metrics for imaging and image processing of multi-views in light sheet fluorescent 3D microscopy. Various metrics of focus are tested on real and simulated data so as to automatically assess the informational quality of the images. Application of such metrics are given for several information tasks including online control of acquisition, fast registration or image fusion. Illustrations are given for typical samples of interest for in vivo imaging with light sheet microscopy such as spheroids or organoids. We point to the reader softwares freely available under FIJI which enable to test the computation of a basic quality metric, for registration and fusion.

Citation Download Citation

Rosa Huaman , Charlotte Riviere, Carole Frindel , Andrea Bassi, and David Rousseau "On the use of image quality measures of multi-views in light sheet fluorescence 3D microscopy", Proc. SPIE 10677, Unconventional Optical Imaging, 106772V (24 May 2018); https://doi.org/10.1117/12.2305844

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