In vivo time-series monitoring of dermal collagen fiber during skin burn healing using second-harmonic-generation microscopy

Eiji Hase; Ryosuke Tanaka; Shu-ichiro Fukushima; Takeshi Yasui

doi:10.1117/12.2316748

24 April 2018 In vivo time-series monitoring of dermal collagen fiber during skin burn healing using second-harmonic-generation microscopy

Eiji Hase, Ryosuke Tanaka, Shu-ichiro Fukushima, Takeshi Yasui

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107111B (2018) https://doi.org/10.1117/12.2316748
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Burn healing is a process to repair thermally damaged tissues. Although burn healing has many aspects, it is common for dynamics of collagen fiber to be closely related with burn healing. If such healing process can be visualized from the viewpoint of the collagen dynamics, one may obtain new findings regarding biological repairing mechanisms in the healing process. In this paper, we applied second-harmonic-generation microscopy for in vivo imaging of the healing process in animal skin burn and successfully visualized the decomposition, production, and growth of renewal collagen fibers as a series of time-lapse images in the same subject.

Citation Download Citation

Eiji Hase, Ryosuke Tanaka, Shu-ichiro Fukushima, and Takeshi Yasui "In vivo time-series monitoring of dermal collagen fiber during skin burn healing using second-harmonic-generation microscopy", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111B (24 April 2018); https://doi.org/10.1117/12.2316748

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available