In vivo visualization of dermal collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held second-harmonic-generation microscope

Yuki Ogura; Kosuke Atsuta; Eiji Hase; Takeo Minamikawa; Takeshi Yasui

doi:10.1117/12.2316930

24 April 2018 In vivo visualization of dermal collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held second-harmonic-generation microscope

Yuki Ogura, Kosuke Atsuta, Eiji Hase, Takeo Minamikawa, Takeshi Yasui

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Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107111C (2018) https://doi.org/10.1117/12.2316930
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Second-harmonic-generation (SHG) microscopy is a powerful tool for in vivo monitoring of collagen fibers in human skin. However, its practical use in the dermatological field is still limited due to the bulky and complicated setup. In this paper, we constructed a photonic-crystal-fiber-coupled, hand-held SHG microscope for in vivo monitoring of collagen fibers in human skin. Fiber delivery of ultrashort pulse light was achieved by a large-mode-area photonic-crystal-fiber whereas the SHG microscopy setup was enclosed into a hand-held probe head. The combination of PCF with the hand-held probe head largely enhances the flexibility of measurement sites in the human skin.

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Yuki Ogura, Kosuke Atsuta, Eiji Hase, Takeo Minamikawa, and Takeshi Yasui "In vivo visualization of dermal collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held second-harmonic-generation microscope", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111C (24 April 2018); https://doi.org/10.1117/12.2316930

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KEYWORDS

Optical fibers

Skin

Second-harmonic generation

In vivo imaging

Collagen

Nonlinear optics

Dispersion

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