In situ monitoring of incised wound healing in animal model using second-harmonic-generation and third-harmonic-generation microscopy

Shu-ichiro Fukushima; Yuji Tanaka; Eiji Hase; Kazuma Takeichi; Takeshi Yasui

doi:10.1117/12.2319458

24 April 2018 In situ monitoring of incised wound healing in animal model using second-harmonic-generation and third-harmonic-generation microscopy

Shu-ichiro Fukushima, Yuji Tanaka, Eiji Hase, Kazuma Takeichi, Takeshi Yasui

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107111E (2018) https://doi.org/10.1117/12.2319458
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Precise assessment of wound healing degree is important to avoid infection and achieve good prognosis without remaining of keloid or hypertrophic scars. In this paper, we apply the combination of second-harmonic-generation (SHG) microscopy with third-harmonic-generation (THG) microscopy for incised wound animal model, and visualize collagen dynamics during the wound healing process in time series in situ. Due to the difference of contrast mechanism, SHG image visualizes distribution of dermal collagen fibers whereas THG image visualizes surface of the epidermis. SHG/THG microscopy enable us to track the phase of wound healing for the same subject from the viewpoint of the temporal and spatial dynamic of collagen fibers and the epidermis surface.

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Shu-ichiro Fukushima, Yuji Tanaka, Eiji Hase, Kazuma Takeichi, and Takeshi Yasui "In situ monitoring of incised wound healing in animal model using second-harmonic-generation and third-harmonic-generation microscopy", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111E (24 April 2018); https://doi.org/10.1117/12.2319458

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