Introducing a data-standard for fluorescence microscopy: increasing data quality and fidelity for biological measurements

Maximiliaan Huisman; Mathias Hammer; Carlas Smith; David Grunwald

doi:10.1117/12.2323660

5 September 2018 Introducing a data-standard for fluorescence microscopy: increasing data quality and fidelity for biological measurements

Maximiliaan Huisman, Mathias Hammer, Carlas Smith, David Grunwald

Author Affiliations +

Proceedings Volume 10726, Nanoimaging and Nanospectroscopy VI; 107260A (2018) https://doi.org/10.1117/12.2323660
Event: SPIE Nanoscience + Engineering, 2018, San Diego, California, United States

Abstract

Single-molecule detection provides ever-more powerful tools. Although technological advances have been made, smart image-processing and -analysis strategies are required for quantification, and to combat issues like unspecific background, limited signal, and optical aberrations. These techniques generally increase the usefulness of microscopy data, but the limits to which these results may be interpreted are often poorly quantified. Here, we suggest a meta-data standard for images obtained through fluorescence microscopy that aims to increasing data fidelity, ease future analysis and facilitate objective comparison of different datasets, experimental setups, and essays.

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Maximiliaan Huisman, Mathias Hammer, Carlas Smith, and David Grunwald "Introducing a data-standard for fluorescence microscopy: increasing data quality and fidelity for biological measurements", Proc. SPIE 10726, Nanoimaging and Nanospectroscopy VI, 107260A (5 September 2018); https://doi.org/10.1117/12.2323660

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