Probing membrane receptors with enhanced Raman imaging

Lifu Xiao; Sian Sloan-Dennison; Zachary D. Schultz

doi:10.1117/12.2321300

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5 September 2018 Probing membrane receptors with enhanced Raman imaging

Lifu Xiao, Sian Sloan-Dennison, Zachary D. Schultz

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Proceedings Volume 10726, Nanoimaging and Nanospectroscopy VI; 107260K (2018) https://doi.org/10.1117/12.2321300
Event: SPIE Nanoscience + Engineering, 2018, San Diego, California, United States

Abstract

Our lab has shown that nanoparticles functionalized with short peptides can selectively bind to receptor proteins in vitro. Our results indicate that the Raman signals observed from purified receptors in surface enhanced Raman scattering (SERS) experiments match those observed with tip-enhanced Raman scattering (TERS) experiments performed on membrane receptors in intact cell membranes. Analysis of the observed Raman signals suggest the signals arise from the amino-acids in the protein receptor responsible for binding and recognition of the ligand attached to the nanoparticle probe. Further experiments show the variance in the data correlates with affinity of the nanoparticle probe with a specific receptor. This result illustrates a new approach to studying membrane receptors.

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Lifu Xiao, Sian Sloan-Dennison, and Zachary D. Schultz "Probing membrane receptors with enhanced Raman imaging", Proc. SPIE 10726, Nanoimaging and Nanospectroscopy VI, 107260K (5 September 2018); https://doi.org/10.1117/12.2321300

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