Comparative analysis for microbial photo-inactivation in continuous and partial therapies

Daniel Ortega; Guillermo García-Torales; Liliana Martínez Chávez; Hilda Mercado-Uribe

doi:10.1117/12.2320853

7 September 2018 Comparative analysis for microbial photo-inactivation in continuous and partial therapies

Daniel Ortega, Guillermo García-Torales, Liliana Martínez Chávez, Hilda Mercado-Uribe

Proceedings Volume 10727, UV and Higher Energy Photonics: From Materials to Applications 2018; 107270Q (2018) https://doi.org/10.1117/12.2320853
Event: SPIE Nanoscience + Engineering, 2018, San Diego, California, United States

Abstract

Photodynamic inactivation is a method widely used to eliminate pathogen micro-organisms. This involves the application of different wavelengths and sources. The ai m of this work is to evaluate the inactivation effect of red light on Escherichia coli applying continuous and partial exposures, and compare with the effect produced by a less used radiation: UVA. We measured survival curves by spectrophotometry and obtained cell cultures. Our results show that red light applied together with methylene blue in continuous and interrupted exposures has not inactivation effect on E. coli. However, UVA radiation applied without photosintetizer exhibits a delay in the first phase of replication process with similar consequences on exponential and stationary phases. It is interesting to explore in the future the use of different compounds which could enhance the effect of UVA radiation.

Citation Download Citation

Daniel Ortega, Guillermo García-Torales, Liliana Martínez Chávez, and Hilda Mercado-Uribe "Comparative analysis for microbial photo-inactivation in continuous and partial therapies", Proc. SPIE 10727, UV and Higher Energy Photonics: From Materials to Applications 2018, 107270Q (7 September 2018); https://doi.org/10.1117/12.2320853

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