The construction of genomic libraries in BAC and its practical application and bioinformatic usage

Agnieszka Skarzyńska; Kohei Yagi; Maciej Kiełek; Wojciech Gutman; Wojciech Pląder; Zbigniew Przybecki

doi:10.1117/12.2501371

1 October 2018 The construction of genomic libraries in BAC and its practical application and bioinformatic usage

Agnieszka Skarzyńska, Kohei Yagi, Maciej Kiełek, Wojciech Gutman, Wojciech Pląder, Zbigniew Przybecki

Proceedings Volume 10808, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2018; 1080835 (2018) https://doi.org/10.1117/12.2501371
Event: Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2018, 2018, Wilga, Poland

Abstract

Libraries constructed in bacterial artificial chromosome (BAC) vectors are essential in modern genomics for all organisms such as plants, animals, insects, algae and microbes. Cloning using a bacterial artificial chromosome (BAC) allows to obtain high quality genomic libraries that are used for physical mapping, cytogenetic research, identification and isolation of genes, as well as for gene sequencing and genomic assembly. Here we describe the methodology of BAC library construction, characterization and the newest application and bioinformatics usage in the post-genomic era.

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Agnieszka Skarzyńska, Kohei Yagi, Maciej Kiełek, Wojciech Gutman, Wojciech Pląder, and Zbigniew Przybecki "The construction of genomic libraries in BAC and its practical application and bioinformatic usage", Proc. SPIE 10808, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2018, 1080835 (1 October 2018); https://doi.org/10.1117/12.2501371

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