Feasibility study of investigation of skin at cellular level by digital holographic microscopy

M. Baczewska; M. Kujawińska; E. Skrzypek; D. Śladowski

doi:10.1117/12.2319578

7 September 2018 Feasibility study of investigation of skin at cellular level by digital holographic microscopy

M. Baczewska, M. Kujawińska, E. Skrzypek, D. Śladowski

Proceedings Volume 10834, Speckle 2018: VII International Conference on Speckle Metrology; 108341P (2018) https://doi.org/10.1117/12.2319578
Event: SPECKLE 2018: VII International Conference on Speckle Metrology, 2018, Janów Podlaski, Poland

Abstract

In this paper we propose and implement the systematic approach to investigation of skin tissue based on sequential determination of refractive index and dry mass density for representative skin cells (3T3 fibroblasts and HaCaT keratinocytes) during their growing in a culture from single cells up to formation of a single layer. The main measurement tool is digital holographic microscopy supported by reference measurements performed by holographic tomography. The results of the measurement are presented and discussed.

Citation Download Citation

M. Baczewska, M. Kujawińska, E. Skrzypek, and D. Śladowski "Feasibility study of investigation of skin at cellular level by digital holographic microscopy", Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 108341P (7 September 2018); https://doi.org/10.1117/12.2319578

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