Multi-modal quantitative analysis of HeLa cells using digital holographic microscopy and confocal laser scanning microscopy

Piotr Stępień; Tytus Bernaś; Wojciech Krauze; Hanna Sas-Nowosielska; Malgorzata Kujawińska

doi:10.1117/12.2319588

7 September 2018 Multi-modal quantitative analysis of HeLa cells using digital holographic microscopy and confocal laser scanning microscopy

Piotr Stępień, Tytus Bernaś, Wojciech Krauze, Hanna Sas-Nowosielska, Malgorzata Kujawińska

Author Affiliations +

Proceedings Volume 10834, Speckle 2018: VII International Conference on Speckle Metrology; 108341V (2018) https://doi.org/10.1117/12.2319588
Event: SPECKLE 2018: VII International Conference on Speckle Metrology, 2018, Janów Podlaski, Poland

Abstract

In the paper the advantages of two different microscopic techniques, namely digital holographic microscopy (DHM) and Confocal Laser Scanning Microscopy (CLSM) have been combined with the aim to investigate HeLa cell culture in terms of statistical analysis of area of subcellular structures of HeLa cells and related to them dry mass estimation. To assure the proper statistical representation of the cells both measurements comprised of multiple fields of view (FoVs), stitched together to form two FoVs with overlapping regions. The results suggest a strong linear correlation of nucleoli dry mass to their projection area, a result that is promising in terms of its biological relevance.

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Piotr Stępień, Tytus Bernaś, Wojciech Krauze, Hanna Sas-Nowosielska, and Malgorzata Kujawińska "Multi-modal quantitative analysis of HeLa cells using digital holographic microscopy and confocal laser scanning microscopy", Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 108341V (7 September 2018); https://doi.org/10.1117/12.2319588

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