High resolution line-field SD-OCT with 2.5 kHz frame rate for cellular resolution imaging of biological tissue

Le Han; Zohreh Hosseiaee; Bingyao Tan; Kostadinka Bizheva

doi:10.1117/12.2511686

22 February 2019 High resolution line-field SD-OCT with 2.5 kHz frame rate for cellular resolution imaging of biological tissue

Le Han, Zohreh Hosseiaee, Bingyao Tan, Kostadinka Bizheva

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Proceedings Volume 10867, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII; 108672X (2019) https://doi.org/10.1117/12.2511686
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

A line-field, spectral domain optical coherence tomography (LF-SD-OCT) system was developed for in-vivo, noncontact, cellular resolution imaging of biological tissue. The LF-SD-OCT system utilizes a broadband laser with a spectrum centered at ~790 nm and spectral bandwidth of ~140 nm to achieve 1.8 μm axial and ~5 μm isotropic lateral resolution in biological tissue. A high speed 2D camera was used to achieve frame rate of 2.5k B-scans/s. The system’s SNR was measured to be 92 dB at 100 μm away from the zero-delay line for 2.8 mW optical power incident on the imaged object, with 18 dB roll-off over a scanning range of 1 mm. The LF-SD-OCT system was used to image the cellular structure of cucumber and the cucumber seed where the high spatial resolution was sufficient to resolve cellular nuclei. Then the system was used to image in-vivo human skin (fingertip), where the spiral structures of the sweat glands, as well as a large number of capillaries were observed in the epidermal layer. Images of the healthy human cornea were also acquired from locations near the corneal apex and the periphery and showed the tissue cellular structure and vasculature. Currently, the corneal images were acquired ex-vivo, as we are waiting for ethics clearance to conduct in-vivo corneal imaging studies with the novel LF-SD-OCT system.

Citation Download Citation

Le Han, Zohreh Hosseiaee, Bingyao Tan, and Kostadinka Bizheva "High resolution line-field SD-OCT with 2.5 kHz frame rate for cellular resolution imaging of biological tissue", Proc. SPIE 10867, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII, 108672X (22 February 2019); https://doi.org/10.1117/12.2511686

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KEYWORDS

Image resolution

Imaging systems

Tissue optics

Optical coherence tomography

In vivo imaging

Eye

Point spread functions

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