Towards in vivo preclinical monitoring of multiscale vascular structure-function relationships in resistant breast cancers with an integrated diffuse and nonlinear imaging system (Conference Presentation)

Kavon Karrobi; Anahita Pilvar; Anup Tank; Kshama Doshi; David J. Waxman; Darren Roblyer

doi:10.1117/12.2508872

4 March 2019 Towards in vivo preclinical monitoring of multiscale vascular structure-function relationships in resistant breast cancers with an integrated diffuse and nonlinear imaging system (Conference Presentation)

Kavon Karrobi, Anahita Pilvar, Anup Tank, Kshama Doshi, David J. Waxman, Darren Roblyer

Author Affiliations +

Proceedings Volume 10871, Multimodal Biomedical Imaging XIV; 108710E (2019) https://doi.org/10.1117/12.2508872
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Alterations in tumor microvascular architecture are associated with resistance to several breast cancer therapies, and may be important markers for in vivo detection of resistance. Our goal is to map how micro-scale alterations in tumor vasculature manifest at the tissue level. To this end, we developed a multiscale preclinical imaging technique called Diffuse and Nonlinear Imaging (DNI) that integrates Spatial Frequency Domain Imaging (SFDI) for tissue-level mapping of tumor optical properties and hemodynamics, with Multiphoton Microscopy (MPM) to image tumor microvascular architecture with cellular resolution. Importantly, SFDI measures the same parameters as clinical Diffuse Optical Spectroscopy, providing a pathway to the clinic for microvascular imaging biomarkers. We demonstrated that the dual modality system can be spatially co-registered with high accuracy and precision (≤ 50 µm), and can be matched in optical sampling depth based on wavelength and spatial frequency selection. We also conducted an in vivo DNI study of untreated murine mammary tumors (Py230) in female C57BL/6 mice, and found strong multiscale relationships between tumor oxygen saturation and micro-vessel diameter, as well as deoxyhemoglobin concentrations and micro-vessel length (|Pearson’s ρ| > 0.5, p < 0.05). We carried out in vivo DNI monitoring in two mammary tumor xenograft models grown in BALB/c athymic nude female mice; one model was responsive to Trastuzumab (Herceptin®) (BT474) and the other was resistant (HR6). This presentation will report on characterizing the vascular structure-function relationships with DNI across length scales within each model, and differences in the multiscale vascular relationships between the models.

Conference Presentation

Citation Download Citation

Kavon Karrobi, Anahita Pilvar, Anup Tank, Kshama Doshi, David J. Waxman, and Darren Roblyer "Towards in vivo preclinical monitoring of multiscale vascular structure-function relationships in resistant breast cancers with an integrated diffuse and nonlinear imaging system (Conference Presentation)", Proc. SPIE 10871, Multimodal Biomedical Imaging XIV, 108710E (4 March 2019); https://doi.org/10.1117/12.2508872

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

In vivo imaging

Tumors

Breast cancer

Brain-machine interfaces

Imaging systems

Resistance

Nonlinear optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years