Human brain glioma grading using label free laser-induced fluorescence spectroscopy

Yan Zhou; Cheng-hui Liu; Ke Zhu; Binlin Wu; Xinguang Yu; Gangge Cheng; Mingyue Zhao; Rui Zong; Chunyuan Zhang; Hong Chen; Shenglin Li; Qijun Liang; Mingqian Zhang; Robert R. Alfano

doi:10.1117/12.2511687

4 March 2019 Human brain glioma grading using label free laser-induced fluorescence spectroscopy

Yan Zhou, Cheng-hui Liu, Ke Zhu, Binlin Wu, Xinguang Yu, Gangge Cheng, Mingyue Zhao, Rui Zong, Chunyuan Zhang, Hong Chen, Shenglin Li, Qijun Liang, Mingqian Zhang, Robert R. Alfano

Author Affiliations +

Proceedings Volume 10873, Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis; 108731A (2019) https://doi.org/10.1117/12.2511687
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Laser-induced fluorescence (LIF) technique was used to generate spectral signatures of endogenous fluorophores relevant to the tissue molecular composition changes in human brain glioma tumors. The goal is to study the changes of fluorescence emission spectra from endogenous fluorophores in human brain glioma of different grades, and to find new biomarkers for prognostic optical molecular pathological diagnosis. Two hundred and thirty-seven (237) native fluorescence spectra from 61 subjects were measured using LabRAM HR Evolution micro photoluminescence (PL) system for four grades of glioma tumors in ex-vivo. The differences of four grades of glioma tumors were identified by the characteristic fluorophores fingerprints under the excitation laser wavelength at UV 325nm. To our best knowledge, this is the first report for human brain study using this technique. The fluorescence peaks of biomarkers with major contribution were found, including tryptophan, collagen, elastin, reduced nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD) and phospholipids that play important roles in the cellular energy metabolism and glycolysis pathway. The ratios of peak intensities and the peak positions in fluorescence spectra of may be used to diagnose human brain diseases or to guide biopsy during surgical resection.

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Yan Zhou, Cheng-hui Liu, Ke Zhu, Binlin Wu, Xinguang Yu, Gangge Cheng, Mingyue Zhao, Rui Zong, Chunyuan Zhang, Hong Chen, Shenglin Li, Qijun Liang, Mingqian Zhang, and Robert R. Alfano "Human brain glioma grading using label free laser-induced fluorescence spectroscopy", Proc. SPIE 10873, Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 108731A (4 March 2019); https://doi.org/10.1117/12.2511687

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