Comparison of two- and three-dimensional nuclear to cytoplasm ratios in analyzing lung cancer cell lines

Hsu-Cheng Huang; Shu-Jen Chiang; Shu-Han Wen; Pei-Jung Lee; Huei-Wen Chen; Yang-Fang Chen; Chen-Yuan Dong

doi:10.1117/12.2507400

4 March 2019 Comparison of two- and three-dimensional nuclear to cytoplasm ratios in analyzing lung cancer cell lines

Hsu-Cheng Huang, Shu-Jen Chiang, Shu-Han Wen, Pei-Jung Lee, Huei-Wen Chen, Yang-Fang Chen, Chen-Yuan Dong

Proceedings Volume 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII; 108810V (2019) https://doi.org/10.1117/12.2507400
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Histological examination has been the primary imaging modality in the diagnosis of eases such as cancer. However, since cells are three-dimensional in nature, the use of traditional nucleus to cytoplasm ratio (N/C) in two dimensions does not represent their three-dimensional structures. In this study, we used two-photon microscopy to acquired threedimensional images of normal human lung cell line Beas2B, human lung adenocarcinoma CL1-0 and CL1-5 cell lines. We determined N/C ratios in two- and three-dimensions and found that 2D N/C-ratio is more precise than 3D N/C-ratio in discriminating normal and cancer cells.

Citation Download Citation

Hsu-Cheng Huang, Shu-Jen Chiang, Shu-Han Wen, Pei-Jung Lee, Huei-Wen Chen, Yang-Fang Chen, and Chen-Yuan Dong "Comparison of two- and three-dimensional nuclear to cytoplasm ratios in analyzing lung cancer cell lines", Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108810V (4 March 2019); https://doi.org/10.1117/12.2507400

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