Towards laser-assisted microfluidic-cell transfection

Hans Georg Breunig; Ana Batista; Aisada König; Karsten König

doi:10.1117/12.2509442

4 March 2019 Towards laser-assisted microfluidic-cell transfection

Hans Georg Breunig, Ana Batista, Aisada König, Karsten König

Proceedings Volume 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII; 108811W (2019) https://doi.org/10.1117/12.2509442
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

By short-term irradiation of a cell with focused laser pulses, the cell membrane can be made transiently permeable. This "optoporation" allows for foreign macro molecules to pass this otherwise impenetrable barrier and enter the cell interior enabling a highly efficient and “hands-off” laser-assisted transfection of cells. We intend to combine a precise flow control of cells with the laser-assisted-transfection technique by employing microfluidics to control the cell flow through the laser-cell interaction region. The precise flow control will allow for a high throughput of cells and combine microfluid cell control with the advantages of the highly efficient laser-assisted transfection scheme. The development of the setup is still in progress. Here, the background and key features are outlined.

Citation Download Citation

Hans Georg Breunig, Ana Batista, Aisada König, and Karsten König "Towards laser-assisted microfluidic-cell transfection", Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108811W (4 March 2019); https://doi.org/10.1117/12.2509442

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