Miniaturizing stimulated Raman scattering microscopy for human subject investigation  (Conference Presentation)

Peng Lin; Shufan Chen; Chien-Sheng Liao; Fengyuan Deng; Oliver Joshua Spires; Yi Zhang; Rongguang Liang; Ji-Xin Cheng

doi:10.1117/12.2509106

4 March 2019 Miniaturizing stimulated Raman scattering microscopy for human subject investigation (Conference Presentation)

Peng Lin, Shufan Chen, Chien-Sheng Liao, Fengyuan Deng, Oliver Joshua Spires, Yi Zhang, Rongguang Liang, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108821Q (2019) https://doi.org/10.1117/12.2509106
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Compared with conventional histology, Stimulated Raman scattering (SRS) microscopy provides high specificity, fast speed and label-free histopathological analysis of the lesions by mapping their chemical compositions. However, benchtop SRS microscopy is limited to its bulky size to access the tissues of interest in-vivo inside the human body. To enable SRS in-vivo label-free histology, here, we develop an implantable fiber-scanning SRS endoscope. The endoscope is capable of providing hyperspectral Raman images at C-H and C-D regions. We use a double-clad single-mode fiber to deliver the pump and Stokes femtosecond pulses through the core and collect back-scattering signals through the outer cladding. To remove the nonlinear background induced by the pulse interactions in the fiber, we temporally separate the two pulses by tuning a delay line. We custom-design a micro-objective made of high-dispersive ZnSe glass which enables a simultaneously focusing and recombining the two pulses at spatial and time domains on the sample for excitation. A piezo actuator is designed to resonantly scan the fiber cantilever with spiral patterns. By establishing this technology, we expect the SRS endoscope to have great potential in medical applications such as label-free image-based diagnosis and surgical guidance.

Conference Presentation

Citation Download Citation

Peng Lin, Shufan Chen, Chien-Sheng Liao, Fengyuan Deng, Oliver Joshua Spires, Yi Zhang, Rongguang Liang, and Ji-Xin Cheng "Miniaturizing stimulated Raman scattering microscopy for human subject investigation (Conference Presentation)", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108821Q (4 March 2019); https://doi.org/10.1117/12.2509106

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KEYWORDS

In vivo imaging

Raman scattering

Endoscopy

Endoscopes

Chemical analysis

Microscopy

Hyperspectral imaging

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