Spatial encoded polarization dependent nonlinear optical analysis for local tensors imaging of collagenous tissue

Changqin Ding; James R. W. Ulcickas; Fengyuan Deng; Garth J. Simpson

doi:10.1117/12.2510049

21 February 2019 Spatial encoded polarization dependent nonlinear optical analysis for local tensors imaging of collagenous tissue

Changqin Ding, James R. W. Ulcickas, Fengyuan Deng, Garth J. Simpson

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Proceedings Volume 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI; 108831E (2019) https://doi.org/10.1117/12.2510049
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Rapid local hyperpolarizability tensor imaging of collagenous tissue was achieved with spatially encoded polarization dependent nonlinear optical measurements. Second harmonic generation (SHG) is sensitive to polarization-dependent measurements due to its unique symmetry requirement, providing rich information of local structures for protein crystals and biological tissues. Fast polarization-dependent measurements reduce 1/f noise and suppress motion blur for in vivo imaging. In this work, spatially encoded polarization dependent SHG was used for local hyperpolarizability tensor imaging of z-cut quartz and collagenous tissue by using a single patterned microretarder array (μRA). The μRA was designed with a pattern of half-wave retardance varying spatially in the azimuthal orientation of the fast-axis. When placed in the rear conjugate plane of a beam scanning microscope, the μRA enabled spatial modulation of incident light with polarization states varied at different positions in the field of view. The ‘snapshot’ approach was available for the polarization dependent measurements of a uniform sample so that one image included a complete set of polarization modulation from different pixels. Combining with sample translation, this method was able to recover local hyperpolarizability tensor of non-uniform samples. This strategy was successfully used to extract local nonlinear optical tensors for z-cut quartz and collagenous tissue with good agreements with traditional polarization dependent measurements, providing an alternate approach for fast polarization analysis of collagen tissue with minimal modifications on current beam scanning nonlinear optical systems.

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Changqin Ding, James R. W. Ulcickas, Fengyuan Deng, and Garth J. Simpson "Spatial encoded polarization dependent nonlinear optical analysis for local tensors imaging of collagenous tissue", Proc. SPIE 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, 108831E (21 February 2019); https://doi.org/10.1117/12.2510049

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KEYWORDS

Polarization

Modulation

Second-harmonic generation

Quartz

Collagen

Tissues

Tissue optics

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