Statistical study of blood cell populations by very wide-field bimodal phase/fluorescence imaging

Isaure de Kernier; Stéphanie Bressieux; Nelly Rongeat; Anaïs Ali-Chérif; Sophie Morales; Serge Monneret; Pierre Blandin

doi:10.1117/12.2506531

20 February 2019 Statistical study of blood cell populations by very wide-field bimodal phase/fluorescence imaging

Isaure de Kernier, Stéphanie Bressieux, Nelly Rongeat, Anaïs Ali-Chérif, Sophie Morales, Serge Monneret, Pierre Blandin

Proceedings Volume 10885, Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics; 108850A (2019) https://doi.org/10.1117/12.2506531
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Very wide-field of view imaging can provide statistical data on large cell populations in a single acquisition. In this paper, we describe a multimodal imaging system combining brightfield, phase and fluorescence contrasts. Its greater simplicity and lower cost compared to flow cytometry make it suitable for Point-Of-Care applications. The system’s resolution was characterized on calibrated beads and resolution targets. We illustrate the potential of the single-shot imaging approach in hematology by studying the specific morphologies of white blood cell sub-types. The results suggest that very wide field of view imaging could be an alternative to flow cytometry for some applications in hematology.

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Citation Download Citation

Isaure de Kernier, Stéphanie Bressieux, Nelly Rongeat, Anaïs Ali-Chérif, Sophie Morales, Serge Monneret, and Pierre Blandin "Statistical study of blood cell populations by very wide-field bimodal phase/fluorescence imaging", Proc. SPIE 10885, Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics, 108850A (20 February 2019); https://doi.org/10.1117/12.2506531

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