Nanocapsule induced morphology and migration changes in single cell layers quantified with digital holographic microscopy

Mathias Kaiser; Luisa Pohl; Steffi Ketelhut; Lena Kastl; Christian Gorzelanny; Martin Götte; Jürgen Schnekenburger; Francisco M. Goycoolea; Björn Kemper

doi:10.1117/12.2509214

4 March 2019 Nanocapsule induced morphology and migration changes in single cell layers quantified with digital holographic microscopy

Mathias Kaiser, Luisa Pohl, Steffi Ketelhut, Lena Kastl, Christian Gorzelanny, Martin Götte, Jürgen Schnekenburger, Francisco M. Goycoolea, Björn Kemper

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Proceedings Volume 10887, Quantitative Phase Imaging V; 108871F (2019) https://doi.org/10.1117/12.2509214
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

We applied quantitative phase imaging (QPI) for quantification of nanocapsule-induced morphology and migration changes in single cell layers. In time-lapse observations, cells were monitored with a Mach-Zehnder interferometerbased off-axis digital holographic microscopy (DHM) setup. For quantification of cell migration, single cells were tracked in the recorded series of quantitative DHM phase images. Moreover, QPI images were evaluated as novel stainfree assay to quantify the temporal course of global cellular morphology changes. The label-free acquired data shows that capsaicin-loaded and unloaded chitosan nanocapsules, and also free capsaicin, significantly influence the direction of cell migration and cellular motility.

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Mathias Kaiser, Luisa Pohl, Steffi Ketelhut, Lena Kastl, Christian Gorzelanny, Martin Götte, Jürgen Schnekenburger, Francisco M. Goycoolea, and Björn Kemper "Nanocapsule induced morphology and migration changes in single cell layers quantified with digital holographic microscopy ", Proc. SPIE 10887, Quantitative Phase Imaging V, 108871F (4 March 2019); https://doi.org/10.1117/12.2509214

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