Viscoelastic mapping of biological samples using Brillouin microscopy (Conference Presentation)

Raimund Schlüßler; Stephanie Möllmert; Shada Abuhattum; Gheorghe Cojoc; Kim Kyoohyun; Paul Müller; Jürgen Czarske; Jochen Guck

doi:10.1117/12.2513291

4 March 2019 Viscoelastic mapping of biological samples using Brillouin microscopy (Conference Presentation)

Raimund Schlüßler, Stephanie Möllmert, Shada Abuhattum, Gheorghe Cojoc, Kim Kyoohyun, Paul Müller, Jürgen Czarske, Jochen Guck

Author Affiliations +

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 108901H (2019) https://doi.org/10.1117/12.2513291
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The viscoelastic material properties of biological systems are increasingly recognized as important parts of signaling cascades involved in developmental and pathological processes. They are furthermore assumed to play a crucial role in surviving extreme environmental conditions for certain organisms, such as yeast cells. Confocal Brillouin microscopy gives access to the viscoelastic material properties of single cells and tissues in a contact- and label-free manner and with a high spatial resolution. In combination with quantitative phase imaging, it is then possible to determine the longitudinal modulus and the viscosity of the sample. In this study, we probed living zebrafish larvae in all anatomical planes, at different time points during development and after spinal cord injury. We could show, that confocal Brillouin microscopy detects the viscoelasticity of different anatomical structures without affecting the animal’s development. We furthermore observed a transiently decreasing Brillouin shift after spinal cord injury and a difference in Brillouin shift between in vivo and ex vivo measurements of the same sample region. Using quantitative phase imaging we additionally show, that the Brillouin shift of the probed tissues is mainly governed by their longitudinal modulus and viscosity. In conclusion, this work constitutes the methodical basis to identify key determinants of viscoelastic tissue properties during biologically important processes in vivo.

Conference Presentation

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Raimund Schlüßler, Stephanie Möllmert, Shada Abuhattum, Gheorghe Cojoc, Kim Kyoohyun, Paul Müller, Jürgen Czarske, and Jochen Guck "Viscoelastic mapping of biological samples using Brillouin microscopy (Conference Presentation)", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108901H (4 March 2019); https://doi.org/10.1117/12.2513291

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KEYWORDS

Microscopy

Confocal microscopy

Tissues

In vivo imaging

Injuries

Phase imaging

Signal processing

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