Nanostructured silicon platform for detection of specific gene sequences

Melania Banu; Monica Simion; Marian Popescu; Lorand Savu

doi:10.1117/12.2324680

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31 December 2018 Nanostructured silicon platform for detection of specific gene sequences

Melania Banu, Monica Simion, Marian Popescu, Lorand Savu

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Proceedings Volume 10977, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies IX; 109773F (2018) https://doi.org/10.1117/12.2324680
Event: Advanced Topics in Optoelectronics, Microelectronics and Nanotechnologies IX, 2018, Constanta, Romania

Abstract

The use of glass supports in microarray technology is favored by their chemical stability, low level of autofluorescence and excellent functionalization capabilities. However, the flat surfaces generate steric hindrance during the hybridization reaction, hence reducing the signal detection in genotyping applications. Herein, a new microarray platform based on nanostructured Si was designated to be used for detection of specific gene fragments corresponding to HPV strains. We investigated the contribution on detection of the functional groups deriving from the chemical surface modification, combined with the effects of nanostructured Si. Uniform spot shapes were associated to surface wetting behaviour and the immobilization and hybridization data were evaluated by fluorescence analyses.

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Melania Banu, Monica Simion, Marian Popescu, and Lorand Savu "Nanostructured silicon platform for detection of specific gene sequences", Proc. SPIE 10977, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies IX, 109773F (31 December 2018); https://doi.org/10.1117/12.2324680

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