Active 3D fluorescence imaging based on holography

Osamu Matoba; Xiangyu Quan; Manoj Kumar; Yasuhiro Awatsuji

doi:10.1117/12.2521022

4 June 2019 Active 3D fluorescence imaging based on holography

Osamu Matoba, Xiangyu Quan, Manoj Kumar, Yasuhiro Awatsuji

Proceedings Volume 10997, Three-Dimensional Imaging, Visualization, and Display 2019; 109970C (2019) https://doi.org/10.1117/12.2521022
Event: SPIE Defense + Commercial Sensing, 2019, Baltimore, MD, United States

Abstract

3D fluorescence imaging based on incoherent digital holography is very useful in the biological field. We have proposed a common-path and off-axis incoherent digital holography using a diffraction grating embedded with a lens to make the self-interference pattern. Based on the observation of the fluorescence image, active illumination of the holographic method is more efficient to avoid unwanted exposure to another area. We present the feasibility experiment to show the potential characteristics of our proposed system.

Citation Download Citation

Osamu Matoba, Xiangyu Quan, Manoj Kumar, and Yasuhiro Awatsuji "Active 3D fluorescence imaging based on holography", Proc. SPIE 10997, Three-Dimensional Imaging, Visualization, and Display 2019, 109970C (4 June 2019); https://doi.org/10.1117/12.2521022

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available