3D visualization in multifocus fluorescence microscopy

Julia R. Alonso; Alejandro Silva; Miguel Arocena

doi:10.1117/12.2520067

14 May 2019 3D visualization in multifocus fluorescence microscopy

Julia R. Alonso, Alejandro Silva, Miguel Arocena

Proceedings Volume 10997, Three-Dimensional Imaging, Visualization, and Display 2019; 109970Q (2019) https://doi.org/10.1117/12.2520067
Event: SPIE Defense + Commercial Sensing, 2019, Baltimore, MD, United States

Abstract

Limited depth-of-field can be overcome through computational optical imaging. In this work, a custom built fluorescence microscope with a focus electrically tunable lens is used for the acquisition of a multifocus image sequence (z-stack) of a 3D fluorescent sample. Image registration between the acquired images is often needed as a preprocessing step before the reconstruction of images with new characteristics. Then a multifocus image fusion algorithm is implemented to accomplish all-in-focus image reconstruction from the registered z-stack. Also computational perspective shifts that allow to the reconstruction of stereoscopic pairs of the sample and its three-dimensional visualization are implemented.

Citation Download Citation

Julia R. Alonso, Alejandro Silva, and Miguel Arocena "3D visualization in multifocus fluorescence microscopy", Proc. SPIE 10997, Three-Dimensional Imaging, Visualization, and Display 2019, 109970Q (14 May 2019); https://doi.org/10.1117/12.2520067

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available