Colorimetric system for paper-based assays

Demetre Liakos; Suzanne Smith; Trudi-Heleen Joubert

doi:10.1117/12.2502538

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24 January 2019 Colorimetric system for paper-based assays

Demetre Liakos, Suzanne Smith, Trudi-Heleen Joubert

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Proceedings Volume 11043, Fifth Conference on Sensors, MEMS, and Electro-Optic Systems; 110430J (2019) https://doi.org/10.1117/12.2502538
Event: Fifth Conference on Sensors, MEMS, and Electro-Optic Systems, 2018, Skukuza, South Africa

Abstract

Paper-based biosensors are used in the field for their low cost and disposability. Their results are often Boolean, however quantitative results would improve derived outcomes. Devices exist which use colorimetry or spectroscopy to extract this data, however they are often bulky, expensive, and specific to the type of assay being performed. The aim of this project was to design a low-cost, portable, and easy to use image-sensing device to optically capture and log results from activated paper-based assays. Markings printed on the assays aid with detection of the assay type. This provides a system extensible to assays of various shapes and sizes. New assays can easily be created and uploaded to the device. Measurements can be taken from up to 30 cm away from the sensor. Once captured, the reaction areas are identified, and the colour intensities are measured to a resolution of 7 bits. Calibration curves are then used to convert these intensities to appropriate units.

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Demetre Liakos, Suzanne Smith, and Trudi-Heleen Joubert "Colorimetric system for paper-based assays", Proc. SPIE 11043, Fifth Conference on Sensors, MEMS, and Electro-Optic Systems, 110430J (24 January 2019); https://doi.org/10.1117/12.2502538

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