CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 11070 > Article
Presentation
14 August 2019 Antimicrobial photodynamic therapy mediated by chlorin e-6 photosensitizer against Streptococcus mutans biofilm (Conference Presentation)
Alessandra Nara de Souza Rastelli, Adilson César Abreu Bernardi, Camila Rodero, Marlus Chorilli, Kléber Thiago de Oliveira, Dongmei Deng, Vanderlei S. Bagnato
Author Affiliations +
Proceedings Volume 11070, 17th International Photodynamic Association World Congress; 110700N (2019) https://doi.org/10.1117/12.2528235
Event: 17th International Photodynamic Association World Congress, 2019, Cambridge, Massachusetts, United States
Abstract
Streptococcus mutans is recognized as the major causative agent of dental caries, especially in fissures and on smooth surfaces. Thus, the purpose of this study was to evaluate the susceptibility of S. mutans (ATCC 25175) biofilm to photodynamic therapy after sensitization with chlorin e-6 (PS) at 0.6 and 2.5μM by exposure to red light at 660nm (BioTable®) under 36.1mW, 15J/cm2 and 5 min of pre-irradiation time. Biofilm was induced in BHI broth supplemented with 1% sucrose for 7 days in a 96 well-plate. Serial dilutions were seeded onto brain heart infusion agar to determine viability in colony-forming units per milliliter (CFU/mL). Additionally, the metabolism of the biofilm by XTT and confocal laser scanning microscopy (CLSM) was performed using BacLight LIVE/DEAD system. Different groups were analyzed: L-D- (negative control), L-D+ (drug), L+D- (light), L+D+ (PDT) and clhorexidine at 0.2% (positive control). Results were analyzed by two-way ANOVA and Tukey’s test (p<0.05). Biofilms were taken for observation at confocal microscopy, and qualitative analyzes were obtained as to the distribution of non-viable viable/cells using LIVE/DEAD® Baclight™ before and after treatments. For CFU/mL and metabolism, the data were submitted to analysis of variance (ANOVA) and Tukey's test at 5% significance. It was observed that there was statistically significant difference in all PDT and chlorhexidine Groups compared to negative control and light Group (p<0.05). No dark cytotoxicity was observed (p>0.05). PDT using chlorin e-6 as photosensitizer can be an adjunct and effective method to control Streptococcus mutans biofilm responsible to dental caries.
Conference Presentation
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Alessandra Nara de Souza Rastelli, Adilson César Abreu Bernardi, Camila Rodero, Marlus Chorilli, Kléber Thiago de Oliveira, Dongmei Deng, and Vanderlei S. Bagnato "Antimicrobial photodynamic therapy mediated by chlorin e-6 photosensitizer against Streptococcus mutans biofilm (Conference Presentation)", Proc. SPIE 11070, 17th International Photodynamic Association World Congress, 110700N (14 August 2019); https://doi.org/10.1117/12.2528235
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Photodynamic therapy
Dental caries
Mode conditioning cables
Statistical analysis
Biological research
Brain
Confocal laser scanning microscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top