Rapid intraoperative margin assessment by using multi-modal third-harmonic generation and three-photon fluorescence microscopy

Chi-Kuang Sun; Chien-Ting Kao; Yi-Hua Liao M.D.; Ming-Liang Wei

doi:10.1117/12.2525658

19 July 2019 Rapid intraoperative margin assessment by using multi-modal third-harmonic generation and three-photon fluorescence microscopy

Chi-Kuang Sun, Chien-Ting Kao, Yi-Hua Liao M.D., Ming-Liang Wei

Author Affiliations +

Proceedings Volume 11073, Clinical and Preclinical Optical Diagnostics II; 110730X (2019) https://doi.org/10.1117/12.2525658
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Slide-free imaging of hematoxylin-eosin-stained whole mount tissues is demonstrated using multi-modal third harmonic generation and three photon fluorescence microscopy. As a result, the demonstrated microscopy shows promise for rapid margin assessment during surgery without compromising the contrast and features of conventional H&E histology.

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Chi-Kuang Sun, Chien-Ting Kao, Yi-Hua Liao M.D., and Ming-Liang Wei "Rapid intraoperative margin assessment by using multi-modal third-harmonic generation and three-photon fluorescence microscopy", Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110730X (19 July 2019); https://doi.org/10.1117/12.2525658

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KEYWORDS

Microscopy

Tissues

Pathology

Tumors

Surgery

Confocal microscopy

Harmonic generation

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