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22 July 2019 Nonlinear imaging of female breast tissue biopsies
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Abstract
In this study nonlinear microscopy techniques utilized to shed new light in breast cancer diagnosis. In particular, the nonlinear imaging modalities of multi-photon excitation fluorescence (MPEF) second and third harmonic generation (SHG, THG) used as non-destructive, label free diagnostic tools for the qualitative discrimination of breast cancer tissues. Nonlinear signals were collected from unstained thin histological sections of breast biopsies samples. In an attempt to discriminate the different types of tissues, quantitative analysis of the recorded THG signals on specific cells in tissue was also performed.
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Evangelia Gavgiotaki, Vassilis Tsafas, Savvas Bovasianos, Sofia Agelaki, Vassilis Georgoulias, Maria Tzardi, Irene Athanassakis, and George Filippidis "Nonlinear imaging of female breast tissue biopsies", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760I (22 July 2019); https://doi.org/10.1117/12.2526285
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