Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy

Björn Kemper; Luisa Pohl; Mathias Kaiser; Eva Döpker; Jürgen Schnekenburger; Steffi Ketelhut

doi:10.1117/12.2527189

22 July 2019 Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy

Björn Kemper, Luisa Pohl, Mathias Kaiser, Eva Döpker, Jürgen Schnekenburger, Steffi Ketelhut

Author Affiliations +

Proceedings Volume 11076, Advances in Microscopic Imaging II; 110760T (2019) https://doi.org/10.1117/12.2527189
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

For the example of digital holographic microscopy (DHM), a variant of quantitative phase imaging (QPI), we explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images. The applicability of the proposed numerical evaluation procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. Our results show that histogram-based evaluation of quantitative phase images enables a highly reliable quantitative observation of global cellular morphology changes in confluent cell layers.

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Björn Kemper, Luisa Pohl, Mathias Kaiser, Eva Döpker, Jürgen Schnekenburger, and Steffi Ketelhut "Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760T (22 July 2019); https://doi.org/10.1117/12.2527189

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