Two-photon excited polarized fluorescence in NADH in methanol/water solutions

I. A. Gorbunova; N. O. Bezverkhnii; A. A. Zhikhoreva; Y. M. Beltukov; M. E. Sasin; J. Rubayo-Soneira; O. S. Vasyutinskii

doi:10.1117/12.2536641

22 July 2019 Two-photon excited polarized fluorescence in NADH in methanol/water solutions

I. A. Gorbunova, N. O. Bezverkhnii, A. A. Zhikhoreva, Y. M. Beltukov, M. E. Sasin, J. Rubayo-Soneira, O. S. Vasyutinskii

Author Affiliations +

Proceedings Volume 11076, Advances in Microscopic Imaging II; 1107621 (2019) https://doi.org/10.1117/12.2536641
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

We studied polarized fluorescence in biological coenzyme NADH in water-methanol solutions upon two-photon excitation with femtosecond laser pulses at 720 nm. The polarized fluorescence decay was recorded by a time correlated single photon counting (TCSPC) system. Fluorescence decay times, rotational diffusion time, fluorescence anisotropy, and the ratio of two pre-exponential factors have been determined and studied as a function of methanol concentration. The results obtained were interpreted on the basis of a model of NADH denaturation processes in solutions and can be used for modeling of NADH binding with various dehydrogenases in living cells.

Citation Download Citation

I. A. Gorbunova, N. O. Bezverkhnii, A. A. Zhikhoreva, Y. M. Beltukov, M. E. Sasin, J. Rubayo-Soneira, and O. S. Vasyutinskii "Two-photon excited polarized fluorescence in NADH in methanol/water solutions", Proc. SPIE 11076, Advances in Microscopic Imaging II, 1107621 (22 July 2019); https://doi.org/10.1117/12.2536641

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available