Near-real time monitoring of bacterial viability using the optrode: a portable fluorimeter

Fang Ou; Julia Robertson; Cushla McGoverin; Simon Swift; Frédérique Vanholsbeeck

doi:10.1117/12.2525558

22 July 2019 Near-real time monitoring of bacterial viability using the optrode: a portable fluorimeter

Fang Ou, Julia Robertson, Cushla McGoverin, Simon Swift, Frédérique Vanholsbeeck

Proceedings Volume 11079, Medical Laser Applications and Laser-Tissue Interactions IX; 1107916 (2019) https://doi.org/10.1117/12.2525558
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

A rapid and easy method for the monitoring of both live and dead bacteria in a sample is valuable in many fields of microbiological and pharmacodynamics studies, and for the monitoring of food safety and public health. Efficient, culture-independent detection of live and dead bacteria can be achieved using differentially staining fluorescent dyes SYTO 9 and propidium iodide (PI). Fluorescence microscopy and flow cytometry have been used extensively for detection of these live/dead cell fluorescence signals, however, both these methods require bulky equipment and are relatively expensive to implement. We are optimising a method to determine live and dead bacterial concentration that takes advantage of an inexpensive fibre-based fluorimeter, the optrode, which can measure fluorescence intensity in bacterial solutions in challenging working environments. The concentrations of live and dead bacteria were predicted using multivariate analysis of optrode-measured fluorescence spectra, which is then compared with results obtained from the flow cytometry measurements. The findings from this study will be used to establish a general method for the monitoring of live and dead bacteria using the optrode.

Citation Download Citation

Fang Ou, Julia Robertson, Cushla McGoverin, Simon Swift, and Frédérique Vanholsbeeck "Near-real time monitoring of bacterial viability using the optrode: a portable fluorimeter", Proc. SPIE 11079, Medical Laser Applications and Laser-Tissue Interactions IX, 1107916 (22 July 2019); https://doi.org/10.1117/12.2525558

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