Video Monitoring And Analysis Subsystem: A CCD-Based Monitoring System For The Protein Crystal Growth Apparatus

William M. Zuk; Keith B. Ward; Mary Ann Perozzo

doi:10.1117/12.960958

26 October 1989 Video Monitoring And Analysis Subsystem: A CCD-Based Monitoring System For The Protein Crystal Growth Apparatus

William M. Zuk, Keith B. Ward, Mary Ann Perozzo

Author Affiliations +

Proceedings Volume 1118, Space Optical Materials and Space Qualification of Optics; (1989) https://doi.org/10.1117/12.960958
Event: SPIE 1989 Technical Symposium on Aerospace Sensing, 1989, Orlando, FL, United States

Abstract

Of significant importance to the field of protein crystallography is the preparation of single crystals suitable for x-ray diffraction studies. Recent experiments have been conducted on the Space Shuttle to study in detail the effects of microgravity on protein crystallization. The device used on these Shuttle missions, the Protein Crystal Growth Apparatus (PCGA), utilizes the vapor diffusion crystallization method and has yielded extremely favorable results. We describe here our efforts to modify the PCGA in order to incorporate an automated CCD-based video system, the Video Monitoring and Analysis Subsystem (VMAS) for direct monitoring of crystallization experiments. The video system discussed allows frequent monitoring and observation of crystal growth. In addition, images of crystallization experiments obtained with the VMAS will be processed and analyzed on-board with a dedicated microcomputer to automatically determine the presence and rate of crystal growth.

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William M. Zuk, Keith B. Ward, and Mary Ann Perozzo "Video Monitoring And Analysis Subsystem: A CCD-Based Monitoring System For The Protein Crystal Growth Apparatus", Proc. SPIE 1118, Space Optical Materials and Space Qualification of Optics, (26 October 1989); https://doi.org/10.1117/12.960958

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