Fluorescence optical super-resolution imaging of nitrogen-vacancy centers based on saturated competition microscopy

Chuankang Li; Yuhang Li; Cuifang Kuang; Xu Liu

doi:10.1117/12.2537518

19 November 2019 Fluorescence optical super-resolution imaging of nitrogen-vacancy centers based on saturated competition microscopy

Chuankang Li, Yuhang Li, Cuifang Kuang, Xu Liu

Author Affiliations +

Proceedings Volume 11186, Advanced Optical Imaging Technologies II; 1118603 (2019) https://doi.org/10.1117/12.2537518
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Super-resolution optical microscopy has enabled the observation of ultra-fine structures and features beyond the diffraction limit, among which nonlinear absorption has been a useful tool to investigate physical and biological characteristics in sub-100 nanometer range. Saturation competition microscopy (SAC), based on nonlinear absorption principle, has been demonstrated to obtain high resolution in either fluorescence or non-fluorescence imaging of biological applications. Furthermore, fluorescent nanodiamonds (FNDs) have been widely researched as nitrogenvacancy centers (NVCs) in FNDs are important medium in quantum entanglement. Here, we report on the characterization of NVCs using pulse SAC (pSAC) microscopy. Resolution of λ/6 has been reached and the experimental results shows that it has better signal-to-background ratio (SBR) with lower illumination intensity in contrast to stimulated emission depletion microscopy (STED).

Citation Download Citation

Chuankang Li, Yuhang Li, Cuifang Kuang, and Xu Liu "Fluorescence optical super-resolution imaging of nitrogen-vacancy centers based on saturated competition microscopy", Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 1118603 (19 November 2019); https://doi.org/10.1117/12.2537518

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