High-speed in vitro intensity diffraction tomography

Jiaji Li; Alex Matlock; Yunzhe Li; Qian Chen; Chao Zuo; Lei Tian

doi:10.1117/12.2536918

19 November 2019 High-speed in vitro intensity diffraction tomography

Jiaji Li, Alex Matlock, Yunzhe Li, Qian Chen, Chao Zuo, Lei Tian

Proceedings Volume 11186, Advanced Optical Imaging Technologies II; 111860Y (2019) https://doi.org/10.1117/12.2536918
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

We demonstrate a label-free, scan-free intensity diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume 3D refractive index distributions in vitro. By optimally matching the illumination geometry to the microscope pupil, our technique reduces the data requirement by 60x to achieve high-speed 10 Hz volume rates. Using 8 intensity images, we recover ∼350 x 100 x 20μm³ volumes with near diffraction-limited lateral resolution of 487 nm and axial resolution of 3.4μm. Based on the 3D quantitative reconstructions of unicellular diatom microalgae, epithelial buccal cell clusters with native bacteria, and live Caenorhabditis elegans specimens, aIDT shows promise as a powerful high-speed, label-free computational microscopy technique for these applications where natural imaging is required to evaluate environmental effects on a sample in real-time.

Citation Download Citation

Jiaji Li, Alex Matlock, Yunzhe Li, Qian Chen, Chao Zuo, and Lei Tian "High-speed in vitro intensity diffraction tomography", Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 111860Y (19 November 2019); https://doi.org/10.1117/12.2536918

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