Axial resolution enhancement in light sheet microscopy using mode modulation

Chengfeng Zhang; Yuchen Chen; Cuifang Kuang; Xu Liu

doi:10.1117/12.2537528

19 November 2019 Axial resolution enhancement in light sheet microscopy using mode modulation

Chengfeng Zhang, Yuchen Chen, Cuifang Kuang, Xu Liu

Proceedings Volume 11186, Advanced Optical Imaging Technologies II; 1118616 (2019) https://doi.org/10.1117/12.2537528
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Light sheet fluorescence microscopy (LSFM) is widely used in biological imaging because of its low photobleaching and phototoxicity. The axial resolution of LSFM is determined and also limited by the thickness of the light sheet and the numerical aperture (NA) of the detection objective. We propose a novel method, light sheet modulation fluorescence microscopy (LSMFM) which is able to achieve a promising axial resolution enhancement of light sheet microscopy.

Citation Download Citation

Chengfeng Zhang, Yuchen Chen, Cuifang Kuang, and Xu Liu "Axial resolution enhancement in light sheet microscopy using mode modulation", Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 1118616 (19 November 2019); https://doi.org/10.1117/12.2537528

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available