Ultrafast time-encoded flow imaging for Giardia cysts and Cryptosporidium oocysts detection

Yingxue Guo; Wanyue Zhao; Xiaohong Zhou; Yun Lu; Minghua Chen; Sigang Yang; Hongwei Chen

doi:10.1117/12.2536789

20 November 2019 Ultrafast time-encoded flow imaging for Giardia cysts and Cryptosporidium oocysts detection

Yingxue Guo, Wanyue Zhao, Xiaohong Zhou, Yun Lu, Minghua Chen, Sigang Yang, Hongwei Chen

Proceedings Volume 11192, Real-time Photonic Measurements, Data Management, and Processing IV; 111920S (2019) https://doi.org/10.1117/12.2536789
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

The harmful effects of Cryptosporidium oocysts and Giardia cysts in drinking water have been widely concerned by the international community. Currently, the EPA1623 method is one of the most mature and authoritative methods for detecting Cryptosporidium oocysts and Giardia cysts internationally. However, this method has the limitations of high cost of time and human labor. Based on ultrashort pulse time-space-frequency mapping principle, ultrafast time-encoded flow imaging can reach high speed and high resolution. Therefore, it is proposed for replacing the last three steps of EPA1623, which are immunomagnetic separation, fluorescent staining and enumeration. Specifically, mixed with immunomagnetic beads, the liquid quality sample of Giardia cysts and Cryptosporidium oocysts flow through the microfluidic channel with high throughput of 100 particles/s. With ultrafast time-encoded flow imaging system, images are acquired including oocysts and cysts which are magnetized by attachment of magnetic beads or not, and only magnetic beads. Extracted appearance and shape features, images are classified by K-means cluster algorithm. It is shown in results that, ultrafast time-encoded flow imaging method costs less than 10 minutes and maintains recovery at more than 80%, compared to the last three steps in EPA1623 which need almost 2 hours at less recovery. The proposed method makes full use of the biological properties of immunomagnetic beads, Cryptosporidium oocysts and Giardia cysts, and maintains high percent recovery with much shorter detection time.

Citation Download Citation

Yingxue Guo, Wanyue Zhao, Xiaohong Zhou, Yun Lu, Minghua Chen, Sigang Yang, and Hongwei Chen "Ultrafast time-encoded flow imaging for Giardia cysts and Cryptosporidium oocysts detection", Proc. SPIE 11192, Real-time Photonic Measurements, Data Management, and Processing IV, 111920S (20 November 2019); https://doi.org/10.1117/12.2536789

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Ultrafast imaging

Magnetism

Feature extraction

Image resolution

Imaging systems

Liquids

Microfluidics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years