High precision pH measurements in biological environments using a portable optical fibre pH sensor

Georgina M. Sylvia; Erik P. Schartner; Hanna J. McLennan; Avishkar Saini; Kylie R. Dunning; Malcolm S. Purdey; Andrew D. Abell; Jeremy G. Thompson

doi:10.1117/12.2541300

30 December 2019 High precision pH measurements in biological environments using a portable optical fibre pH sensor

Georgina M. Sylvia, Erik P. Schartner, Hanna J. McLennan, Avishkar Saini, Kylie R. Dunning, Malcolm S. Purdey, Andrew D. Abell, Jeremy G. Thompson

Author Affiliations +

Proceedings Volume 11202, Biophotonics Australasia 2019; 112020F (2019) https://doi.org/10.1117/12.2541300
Event: ANZCOP, 2019, Melbourne, Australia

Abstract

We have demonstrated that an optical fibre-based pH sensor can be utilised to accurately assess pH in a biological environment. Initial measurements were performed on 5 μL drops of culture medium containing individual female mouse reproductive cells (cumulus-oocyte-complexes, COCs), with the goal of obtaining a biomarker of individual cell health during assisted reproductive processes. Improvements to the measurement procedure were found to reduce fluorescence signal variability, enabling improved measurement precision compared to previous studies. Results show the application of treatments which serve to increase lactic acid production by the COC, and thus induce an acidification of the local microenvironment, are detectable by the pH sensor. This optical technology presents a promising platform for the measurement of pH and the detection of other extracellular biomarkers to assess cell health during assisted reproduction.

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Georgina M. Sylvia, Erik P. Schartner, Hanna J. McLennan, Avishkar Saini, Kylie R. Dunning, Malcolm S. Purdey, Andrew D. Abell, and Jeremy G. Thompson "High precision pH measurements in biological environments using a portable optical fibre pH sensor", Proc. SPIE 11202, Biophotonics Australasia 2019, 112020F (30 December 2019); https://doi.org/10.1117/12.2541300

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