On-site portable single molecule fluorescence imaging device for high-sensitive and accurate target detection

Dan Jian; Zhilong Jiang; Yan Kong; Xiaoliang He; Cheng Liu; Shouyu Wang

doi:10.1117/12.2548833

20 December 2019 On-site portable single molecule fluorescence imaging device for high-sensitive and accurate target detection

Dan Jian, Zhilong Jiang, Yan Kong, Xiaoliang He, Cheng Liu, Shouyu Wang

Author Affiliations +

Proceedings Volume 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019); 112093E (2019) https://doi.org/10.1117/12.2548833
Event: Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 2019, Xi'an, China

Abstract

Single molecule fluorescence detecting technique often has extremely high accuracy and sensitivity, thus it is often adopted in biomedicine detection, pollutant monitoring and chemical analysis. However, traditional single molecule fluorescence detecting devices often suffer from huge sizes and expensive costs, inevitably limiting their applications especially in on-site detections. In order to extend its application scope, we design a single molecule fluorescence imaging device (smFID), in which a laser is tilted illuminated on the sample chip, and a micro-objective is used for fluorescence signal collection, moreover, a smartphone with a filter is adopted to record the fluorescence images. According to the system design, smFID can reach a wide field of view of 1.5mm², a high resolution of 2.2 μm and signal to noise ratio of ~22dB. Additionally, smFID is implemented in single molecule detection combining with the magnetic bead system, and its sensitivity can reach 1 nM and its detection range is wide from 1 nM to 1 μM, which are much better than traditional chemical and optical methods. Considering smFID can be used for single molecule testing with compact configuration, fast speed, high sensitivity and accuracy, it is a favorable device potentially used in on-site single molecule sensing and imaging fields.

Citation Download Citation

Dan Jian, Zhilong Jiang, Yan Kong, Xiaoliang He, Cheng Liu, and Shouyu Wang "On-site portable single molecule fluorescence imaging device for high-sensitive and accurate target detection", Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112093E (20 December 2019); https://doi.org/10.1117/12.2548833

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Molecules

Mercury

Signal detection

Target detection

Signal to noise ratio

Fluorescence spectroscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years