CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 11215 > Article
Presentation
9 March 2020 FLIm guided Raman imaging for detecting bovine pericardium cross-links and calcification (Conference Presentation)
Tanveer Ahmed Shaik, Alba Alfonso Garcia, Xiangnan Zhou, Anne K. Haudenschild, Christoph Krafft, Laura Marcu, Jürgen Popp
Author Affiliations +
Proceedings Volume 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020; 112150I (2020) https://doi.org/10.1117/12.2545632
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
We demonstrated the ability of fiber-based Fluorescence Lifetime Imaging (FLIm) guided Raman spectroscopy to monitor the quality of engineered vascular grafts with high speed and specificity. We report FLIm guided Raman imaging as an effective multimodal technique to evaluate scaffold cross-linking and localized calcification. Current results indicate that the lifetime of AR-BP shortens upon GA cross-linking,and Raman spectroscopy reveals secondary structural changes occurring in the Amide I region of cross-linked pericardia. GA fixed vascular grafts are prone to calcification, an effect linked to graft failure. The calcified regions exhibited shorter lifetimes in fluorescence spectral bands ranging from 380 to 455 nm and Raman spectra exhibited the specific hydroxyapatite signature at 960 cm-1 co-localized with these lower lifetime regions. We conclude that FLIm guided Raman imaging can detect cross-linking signatures and areas of calcification in tissue with biochemical specificity.
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Tanveer Ahmed Shaik, Alba Alfonso Garcia, Xiangnan Zhou, Anne K. Haudenschild, Christoph Krafft, Laura Marcu, and Jürgen Popp "FLIm guided Raman imaging for detecting bovine pericardium cross-links and calcification (Conference Presentation)", Proc. SPIE 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020, 112150I (9 March 2020); https://doi.org/10.1117/12.2545632
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Raman spectroscopy
Analog electronics
Data acquisition
Fluorescence lifetime imaging
Luminescence
Nondestructive evaluation
Target acquisition
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top