Switchable vertical/horizontal section imaging with line-field confocal optical coherence tomography

Jonas Ogien; Olivier Levecq; Hicham Azimani; Arnaud Dubois

doi:10.1117/12.2547454

21 February 2020 Switchable vertical/horizontal section imaging with line-field confocal optical coherence tomography

Jonas Ogien, Olivier Levecq, Hicham Azimani, Arnaud Dubois

Proceedings Volume 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV; 1122816 (2020) https://doi.org/10.1117/12.2547454
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Line-field confocal optical coherence tomography (LC-OCT) is an imaging method based on dynamically-focused line-field time-domain OCT to generate vertical section images of biological tissues with cellular spatial resolution. We demonstrate here an improvement in the LC-OCT technology that makes it possible to also generate horizontal (en face) section images, as in conventional optical coherence microscopy (OCM) or reflectance confocal microscopy (RCM). This improvement is based on the implementation of a lateral scanning device using a mirror galvanometer to scan the laser beam laterally at a given depth. The reference mirror of the interferometer is mounted on a piezoelectric transducer to generate a sinusoidal phase modulation during lateral scanning in order to extract the tomographic signal. The LC-OCT device is capable of generating either a horizontal section image at an adjustable depth in the sample, or a vertical section image at an adjustable lateral position in the sample. For both operating modes, images are acquired in real-time (8 frames per second), with real-time control of the depth/lateral position. The user can switch between the two operating modes by clicking on a button. Using a supercontinuum laser as a light source and 0.5 NA microscope objectives, the image resolution is 0.9 × 0.9 × 1.1 μm³ (x × y × z), with fields of view of 1.2 × 0.5 mm² (x × y, horizontal) and 1.2 × 0.4 mm² (x × z, vertical). In vivo cellular-level imaging of human skin is demonstrated for both modes of operation.

Citation Download Citation

Jonas Ogien, Olivier Levecq, Hicham Azimani, and Arnaud Dubois "Switchable vertical/horizontal section imaging with line-field confocal optical coherence tomography", Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 1122816 (21 February 2020); https://doi.org/10.1117/12.2547454

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Optical coherence tomography

Imaging systems

In vivo imaging

Interferometers

Confocal microscopy

Image resolution

Show All Keywords

Keywords/Phrases

Search In:

Publication Years