Performance requirements for lasers and fibers suited for confocal microscopy and flow cell cytometry applications

Volker Melzer; Richard Addy; Huw Major

doi:10.1117/12.2544290

17 February 2020 Performance requirements for lasers and fibers suited for confocal microscopy and flow cell cytometry applications

Volker Melzer, Richard Addy, Huw Major

Author Affiliations +

Proceedings Volume 11231, Design and Quality for Biomedical Technologies XIII; 1123105 (2020) https://doi.org/10.1117/12.2544290
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The availability of high quality laser diode based lasers in the visible range has boosted up many bio-medical applications like Confocal Microscopy and Flow Cytometry. These new lasers are enabling high resolution close to the diffraction limit and improved sensitivity in many biological and medical applications. We explain the most important performance parameters of these lasers which are required to achieve best test results and excellent resolution by using suited laser sources and accessories for these two applications. Some of these parameters are very low noise, low beam pointing, single TEM00 mode like beam quality for diffraction limited spots and the beam shape. Beside the laser sources the use of single mode fibers is essential to achieve excellent laser beam quality and tailored beam shapes in combination with very low beam pointing for highest resolution and superior test results. The paper will explain the important role of Spatial Filtering and how it is working based on apodization in the Fourier space.

Citation Download Citation

Volker Melzer, Richard Addy, and Huw Major "Performance requirements for lasers and fibers suited for confocal microscopy and flow cell cytometry applications", Proc. SPIE 11231, Design and Quality for Biomedical Technologies XIII, 1123105 (17 February 2020); https://doi.org/10.1117/12.2544290

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