Vascular and functional imaging by a fast mechanical scanning dual-wavelength photoacoustic microscopy (PAM)

Renzhe Bi; Malini Olivo; Yang Pu

doi:10.1117/12.2544270

17 February 2020 Vascular and functional imaging by a fast mechanical scanning dual-wavelength photoacoustic microscopy (PAM)

Renzhe Bi, Malini Olivo, Yang Pu

Author Affiliations +

Proceedings Volume 11240, Photons Plus Ultrasound: Imaging and Sensing 2020; 112403P (2020) https://doi.org/10.1117/12.2544270
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We present a dual-wavelength fast mechanical scanning optical-resolution photoacoustic microscopy system (OR-PAM) in this paper. Conventional mechanical scanning OR-PAM system takes ~ 30 min for one frame of single wavelength imaging. The acquisition of two wavelengths will double the total imaging time. By using a fast-linear stage, a high signal-to-noise (SNR) optical and acoustic combiner and 2 lasers at different wavelengths, we achieved a B-scan rate of 12 Hz and reduced the acquisition time to ~1.5 min for dual-wavelength imaging. This system can be used in large field optical resolution imaging. Contrast-free vascular and functional imaging can be achieved using this system. Both ex vivo and in vivo imaging results are demonstrated.

Citation Download Citation

Renzhe Bi, Malini Olivo, and Yang Pu "Vascular and functional imaging by a fast mechanical scanning dual-wavelength photoacoustic microscopy (PAM)", Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112403P (17 February 2020); https://doi.org/10.1117/12.2544270

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