Vascular and functional imaging by a fast mechanical scanning dual-wavelength photoacoustic microscopy (PAM)

Renzhe Bi; Malini Olivo; Yang Pu

doi:10.1117/12.2544270

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17 February 2020 Vascular and functional imaging by a fast mechanical scanning dual-wavelength photoacoustic microscopy (PAM)

Renzhe Bi, Malini Olivo, Yang Pu

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Proceedings Volume 11240, Photons Plus Ultrasound: Imaging and Sensing 2020; 112403P (2020) https://doi.org/10.1117/12.2544270
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We present a dual-wavelength fast mechanical scanning optical-resolution photoacoustic microscopy system (OR-PAM) in this paper. Conventional mechanical scanning OR-PAM system takes ~ 30 min for one frame of single wavelength imaging. The acquisition of two wavelengths will double the total imaging time. By using a fast-linear stage, a high signal-to-noise (SNR) optical and acoustic combiner and 2 lasers at different wavelengths, we achieved a B-scan rate of 12 Hz and reduced the acquisition time to ~1.5 min for dual-wavelength imaging. This system can be used in large field optical resolution imaging. Contrast-free vascular and functional imaging can be achieved using this system. Both ex vivo and in vivo imaging results are demonstrated.

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Renzhe Bi, Malini Olivo, and Yang Pu "Vascular and functional imaging by a fast mechanical scanning dual-wavelength photoacoustic microscopy (PAM)", Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112403P (17 February 2020); https://doi.org/10.1117/12.2544270

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