Fiber-based instrument for simultaneous exogenous fluorescence and endogenous fluorescence lifetime imaging of engineered vascular tissue (Conference Presentation)

Cai Li; Alba Alfonso García; Lauren Uyesaka; Benjamin E. Sherlock; Leigh G. Griffiths; Laura Marcu

doi:10.1117/12.2545251

9 March 2020 Fiber-based instrument for simultaneous exogenous fluorescence and endogenous fluorescence lifetime imaging of engineered vascular tissue (Conference Presentation)

Cai Li, Alba Alfonso García, Lauren Uyesaka, Benjamin E. Sherlock, Leigh G. Griffiths, Laura Marcu

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 1124313 (2020) https://doi.org/10.1117/12.2545251
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Endogenous and exogenous fluorescence imaging have shown great values for monitoring and studying all kind of biological processes including vascular tissue regeneration and disease progression. In this study, we present a fiber-based optical imaging instrument that is able to simultaneously acquire endogenous and exogenous fluorescence images from tissue samples using a reflective optical chopper wheel to temporally interleave the two modalities. The functionality of the system was demonstrated by imaging native tissue constructs seeded with cells labeled with different dyes. The cellular regions were clearly resolved in both modalities, providing an exogenous and in-situ validation for endogenous fluorescence lifetime images.

Conference Presentation

Citation Download Citation

Cai Li, Alba Alfonso García, Lauren Uyesaka, Benjamin E. Sherlock, Leigh G. Griffiths, and Laura Marcu "Fiber-based instrument for simultaneous exogenous fluorescence and endogenous fluorescence lifetime imaging of engineered vascular tissue (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 1124313 (9 March 2020); https://doi.org/10.1117/12.2545251

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KEYWORDS

Luminescence

Tissues

Imaging systems

Fluorescence lifetime imaging

Microscopy

Multiplexing

Optical imaging

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